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. 2017 Jan 30;12(1):e0170681. doi: 10.1371/journal.pone.0170681

Table 1. Primers used in the quantitative real time PCR.

Target gene Forward sequence (5’-3’) Reverse sequence (5’-3’) GenBank no.
IL-2 GACTACAGCTTATGGAGCACCTCT ACTCCTTTGTGTCATTTGGTGTGT AF294323
IL-6 CCCAGAAATCCCTCCTCACA CAAATAGCGAACAGCCCTCAC JQ728554.1
IFN-α TCCTCCAACACCTCTTCGAC GGGCTGTAGGTGTGGTTCTG DQ861429
IFN-γ TCAGAGACCTCGTGGAACTGTC ACTGGCTCCTTTTCCTTTTGG AJ012254
CD4 GTCCCATCCCACCTAATGTCC CTCCACCTTTGTTTCACTCTGTTTT NM001310403.1
CD8α GAAGTCCTTCAAGGCAGAG AGACGTCCCTCTTGGTGAC JX051841
MHC-I GAAGAGCAAGCAGGGGAAGA TGAAGCAGAGCGGTTAGACAC AB115246.1
MHC-II CTCGAGGTCATGATCAGCAA TGTAAACGTCTCCCCTTTGG AY905540.1
β-Actin TGATGGACTCTGGTGATGGTG ATTTCTCTCTCGGCTGTGGTG EF667345.1