Table 1. Primers used in the quantitative real time PCR.
Target gene | Forward sequence (5’-3’) | Reverse sequence (5’-3’) | GenBank no. |
---|---|---|---|
IL-2 | GACTACAGCTTATGGAGCACCTCT | ACTCCTTTGTGTCATTTGGTGTGT | AF294323 |
IL-6 | CCCAGAAATCCCTCCTCACA | CAAATAGCGAACAGCCCTCAC | JQ728554.1 |
IFN-α | TCCTCCAACACCTCTTCGAC | GGGCTGTAGGTGTGGTTCTG | DQ861429 |
IFN-γ | TCAGAGACCTCGTGGAACTGTC | ACTGGCTCCTTTTCCTTTTGG | AJ012254 |
CD4 | GTCCCATCCCACCTAATGTCC | CTCCACCTTTGTTTCACTCTGTTTT | NM001310403.1 |
CD8α | GAAGTCCTTCAAGGCAGAG | AGACGTCCCTCTTGGTGAC | JX051841 |
MHC-I | GAAGAGCAAGCAGGGGAAGA | TGAAGCAGAGCGGTTAGACAC | AB115246.1 |
MHC-II | CTCGAGGTCATGATCAGCAA | TGTAAACGTCTCCCCTTTGG | AY905540.1 |
β-Actin | TGATGGACTCTGGTGATGGTG | ATTTCTCTCTCGGCTGTGGTG | EF667345.1 |