Fig 4. Production of TGFβ and anti-TGFβ in MPL^WT and MPL^W515L-transduced bone marrow-derived MSC.

(A) Diagram showing conditions used for this experiment. mRNA expression (B) and secreted levels (C) of TGFβ from MSCs derived from mice transplanted with bone marrow expressing MPL^WT or MPL^W515L retroviruses. Collagen I (Col1A1) (D) and III (Col3A1) (E) mRNA expressed in these cells in the presence of anti-TGFβ (1 and 5 μg/ml) monoclonal antibody during a three day culture period. (F) Collagen I (Col1A1) mRNA in MSCs derived from MPL^WT mice under treatment with TGFβ and anti-TGFβ antibody. Relative expression to TATA binding protein (TBP) control is shown. Statistical analysis was performed using 2-sided t test with significance <0.05. Experiments are shown for three biological replicates.