Fig 5. Elevated signaling pathways induced stably in MSCs from animals with MPN.

(A) Western blot analysis with (B) quantification of the bands for pSmad3, Smad3, pSTAT3, STAT3, and β-actin. Shown are cell extracts from WT1 and WT2 cell lines (MSCs derived from bone marrow expressing MPL^WT) and MU1 and MU2 (MSCs derived from bone marrow expressing MPL^W515L). The experimental results are representative of three. Statistical analysis was performed using 2-sided t test with significance <0.05.