Fig 12. Performance of poly(A) site prediction in HSV-1.

(A) Read coverage (= number of read pairs mapped divided by genome length) on the HSV-1 genome for the newly transcribed RNA samples. (B) PPV and sensitivity for poly(A) sites in HSV-1. A poly(A) site was considered a true positive if it was within 50 nt downstream of an annotated poly(A) signal and a false positive otherwise. A poly(A) signal without a predicted poly(A) site within 50 nt downstream was considered a false negative.