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. 2017 Jan 30;12(1):e0171073. doi: 10.1371/journal.pone.0171073

Fig 3. Anti- peptide antibodies bind Klebsiella pneumoniae-derived proteins.

Fig 3

(A) Sequence homology between the AS peptide and the Klebsiella pneumoniae-derived proteins: sugar transporter (SET) protein, Xaa-Pro dipeptidase (DPP) protein and L-fucose isomerase (LFI) protein. Binding of affinity purified anti-AS peptide antibodies (black continuous line) to the SET (B) and LFI (C) peptide in DELFIA. Binding of affinity purified anti-AS peptide antibodies to recombinant DPP protein in ELISA (D). Dotted line: antibodies affinity purified against an irrelevant control peptide. X axis: increasing antibody concentration by two fold ranging from 1.25 microgram/ml to 10 microgram/ml. Y axis: IgG international units for DELFIA assay and Optical Density values obtained at 405 nm wavelength for ELISA assay.