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. Author manuscript; available in PMC: 2017 Jan 30.
Published in final edited form as: Cell Rep. 2016 Jul 28;16(6):1574–1587. doi: 10.1016/j.celrep.2016.07.016

Figure 7. NUC1 interacts with Telomerase and its disruption induces telomeres shortening.

Figure 7

A. TRF analyses of telomere length in 2 WT Col-0 replicates, 2 nuc1 mutant alleles, nuc2-2 or complemented nuc2 mutant, as well as in fas1–4 and fas2–4 mutant. B–E. PETRA analyses of telomere length in 2 nuc1 mutant alleles, 2 replicates of WT Col-0, as well as in fas1–4 and fas2–4 mutants. Chromosome specific probes were used to detect the telomere of the left arm of chromosome 3 (B), the right arm of chromosome 4 (C), as well as the left (D) or right (E) arm of chromosome 5. (F) Telomerase activity was analyzed in fractions obtained during the IP-TRAP experiment using pNUC1-NUC1-FLAG-tagged and WT Col-0 plants. Total input, total protein extract prior to IP; unbound, unbound faction after the capture by ANTI-FLAG magnetic beads; 1st wash, 1st wash of the fraction bound to magnetic beads with buffer W; 2nd wash, 2nd wash of the fraction bound to magnetic beads with buffer W; FLAG-IP, a fraction specifically bound to magnetic beads; denatured, reaction with denatured total protein extract (5 min, 95 °C); -, reaction with buffer W.