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. Author manuscript; available in PMC: 2017 Jan 30.
Published in final edited form as: J Clin Cell Immunol. 2016 Nov 25;7(6):471. doi: 10.4172/2155-9899.1000471

Figure 3.

Figure 3

Expression of CCKAR message in immune organs and PBMC. RNA was extracted from different tissues and from PBMC from four 20 day old birds, and reverse transcribed to cDNA. Amplification was performed using Real time PCR system. Shown are averages +/− SEM of ΔCT values from 4 birds for each breed, calculated by determining the CT value of CCKAR compared to that of the house keeping gene, β actin. Two-way ANOVA followed by two tailed unpaired t-test were used to determine the statistical analysis. ** and *** denotes significance with p<0.01 and p<0.001, respectively.