Table 1.

CCKAR and cytokines primers used in conventional and real time PCR.

A. Primers used in conventional PCR
Forward primer (3 ------ 5)	Reverse primer (5 ------ 3)	TM	Size	Exon/boundary
GTGTGCATATGTCTGAATGTGTG	TTGGGAATGAGGGTGAATGG	64	559	Exon 1 and 2
TGCATGCCATTCACCCTCAT	AATGAGGCCATACGCAACCA	60	417	Exon 2, 3 and 4
GGATAATAATGATGGTTGCGTATGG	GTAGGACAGCAGGTGGATAAAG	64	413	Exon 5
B. Primers used in Real time PCR
Gene ID	Catalog No.	Ref Seq Accession no.	Amplicon Size (bp)	Company
CCKAR	PPG07425A	NM_001081501.1	74	QIAGEN
IL-1β	PPG00540A	NM_204524.1	138	QIAGEN
IL-6	PPG00665C	NM_204628.1	123	QIAGEN
IFN-γ	PPG01291A	NM_205149.1	72	QIAGEN
GAPDH	PPG00285B	NM_204305.1	115	QIAGEN
Actin Beta	F:CGGACTGTTACCAACACCC R:ATCCTGAGTCAAGCGCCAAA	NM_205518.1	116	IDT