Evaluation of insulin signaling in iNOS KO and control mice in response to intraperitoneal insulin. (A) Insulin-induced Protein kinase B (Akt) phosphorylation in liver, epididymal adipose tissue, and gastrocnemius muscle. (B–D) Akt phosphorylation densitometry in (B) liver, (C) epididymal adipose tissue, and (D) gastrocnemius muscle. *p < 0.05 vs. C57BL/6J HFD, ˆp < 0.05 vs. iNOS KO HFD. Bars represent mean ± SEM from 4 to 8 mice. Similar results were observed when using delta percentage of baseline.