Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 30;6:e21392. doi: 10.7554/eLife.21392

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017, Amor et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 4. — (a, b) TEM of longitudinal sections from dorsal roots of Nfasc^fl/fl;Sptbn1^fl/fl (a) and Avil-Cre;Nfasc^fl/fl;Sptbn1^fl/fl mice (b). Scale bars, 0.5 µm. (c, d) TEM of cross sections through dorsal roots of Nfasc^fl/fl;Sptbn1^fl/fl (c) and Avil-Cre;Nfasc^fl/fl;Sptbn1^fl/fl mice (d). Scale bars, 4 µm. (e) Immunostaining of P10 Nfasc^fl/fl;Sptbn1^fl/fl and Avil-Cre;Nfasc^fl/fl;Sptbn1^fl/fl dorsal roots using antibodies against Na⁺ channels and Caspr shows intact paranodal junctions. Scale bar, 5 µm. (f–h) Immunostaining of P15 Nfasc^fl/fl;Sptbn1^fl/fl and Avil-Cre;Nfasc^fl/fl;Sptbn1^fl/fl dorsal roots using antibodies against Na⁺ channels (e, red), AnkG (f, red), βIV spectrin (g, red) and panNF (green). Scale bar, 5 µm. (i) The percentage of nodes labeled for Na⁺ channels in P10 and P15 Nfasc^fl/fl;Sptbn1^fl/fl and Avil-Cre;Nfasc^fl/fl;Sptbn1^fl/fl dorsal and ventral roots. N = 4 animals per age and genotype. ***p=2.0E−06 at P10; p=6.26E−08 at P15.

DOI: http://dx.doi.org/10.7554/eLife.21392.008