Author response image 1.
(A) Indentation of Neuro2A cells. Neuro2A cells were indented using a glass probe and resulting mechanically-gated currents were monitored using whole-cell patch-clamp. Indentation stimuli were applied before, during and after application of GSK205 (10 µM, 3 min; washout 5 min). Current amplitudes were normalised against pre-treatment currents. (B) Indentation experiments were repeated on cells treated with Extracellular solution instead of GSK205. Data represents average ± s.e.m., n = 6 cells (GSK205) vs 5 cells (negative control), from two separate experiments.