Table 1.
FLIM analysis of the PS1 NT-CT proximity in phosphorylation-inhibited PS1 mutants.
Construct |
*Relative FRET efficiency (%) |
p value |
||
|---|---|---|---|---|
DMSO |
A23187 |
‡vs WT (in DMSO) |
§DMSO vs A23187 |
|
PS1 Wild type (WT) |
100 ± 8.9 (n = 18) |
134.7 ± 7.5 (n = 25) |
- |
†p<0.05 |
PS1 S28A |
106.9 ± 7.0 (n = 19) |
143.4 ± 8.6 (n = 19) |
n.s. |
†p<0.05 |
PS1 T74A |
96.5 ± 13.9 (n = 14) |
98.5 ± 8.3 (n = 22) |
n.s. |
n.s. |
PS1 S310A |
88.8 ± 10.2 (n = 17) |
95.1 ± 8.6 (n = 24) |
n.s. |
n.s. |
PS1 S313A |
92.7 ± 8.4 (n = 15) |
108.0 ± 8.8 (n = 21) |
n.s. |
n.s. |
PS1 S310A/S313A |
84.2 ± 6.7 (n = 26) |
87.8 ± 9.0 (n = 17) |
n.s. |
n.s. |
PS1 S319A/T320A |
106.2 ± 8.1 (n = 20) |
131.8 ± 8.1 (n = 20) |
n.s. |
†p<0.05 |
PS1 S324A |
93.8 ± 6.2 (n = 19) |
129.9 ± 8.4 (n = 15) |
n.s. |
†p<0.05 |
PS1 S337A |
99.9 ± 8.8 (n = 13) |
132.4 ± 10.9 (n = 16) |
n.s. |
†p<0.05 |
PS1 S346A |
100.1 ± 8.9 (n = 18) |
135.0 ± 5.2 (n = 19) |
n.s. |
†p<0.05 |
PS1 S353A |
89.6 ± 7.4 (n = 18) |
129.8 ± 8.3 (n = 23) |
n.s. |
†p<0.05 |
PS1 T354A |
74.4 ± 5.6 (n = 14) |
113.4 ± 7.7 (n = 23) |
n.s. |
†p<0.05 |
PS1 S357A |
91.2 ± 7.9 (n = 18) |
119.8 ± 9.2 (n = 23) |
n.s. |
†p<0.05 |
PS1 S353A/S357A |
98.9 ± 9.1 (n = 14) |
125.9 ± 7.1 (n = 13) |
n.s. |
†p<0.05 |
PS1 S365A |
102.3 ± 6.4 (n = 19) |
87.3 ± 9.9 (n = 21) |
n.s. |
n.s. |
PS1 S366A |
102.8 ± 6.7 (n = 16) |
101.6 ± 12.6 (n = 16) |
n.s. |
n.s. |
PS1 S367A |
99.7 ± 6.1 (n = 10) |
99.1 ± 11.4 (n = 15) |
n.s. |
n.s. |
PS1 S365A/S367A |
104.1 ± 6.7 (n = 19) |
102.8 ± 8.4 (n = 19) |
n.s. |
n.s. |
PS1 S366A/S367A |
102.7 ± 11.2 (n = 10) |
108.2 ± 9.9 (n = 19) |
n.s. |
n.s. |
*The FRET efficiency in DMSO-treated cells expressing WT PS1 is set as 100%, and relative FRET efficiency in phosphorylation-inhibited mutants of PS1 is shown. Mean ± SEM, Student’s t-test, n: cell number, †: p<0.05, n.s.: not significant.
‡p-value is shown for the comparison between WT PS1 and phosphorylation-inhibited mutants of PS1 in DMSO-treated conditions, or §for the comparison between DMSO-treated and A23187 (5 µM for 15 min)-treated cells expressing the same PS1 construct.