Figure 6.

JQ1 diminishes renal inflammation in the NTS nephritis. Accelerated NTS nephritis was done in mice by administration of an antimurine glomerular basement membrane rabbit antiserum, and mice were studied 10 days after immunization. Mice were treated or not treated with JQ1 (100 mg/kg per day) starting before the first NTS injection. (A) In paraffin–embedded kidney sections of NTS mice, glomerular infiltration of monocytes/macrophages was found (detected using an antibody against F4/80). B shows the quantification of immunohistochemistry. (C and D) RNA was isolated from frozen samples of whole kidney, and gene expression levels were evaluated by real–time quantitative PCR. (E) JQ1 ameliorates renal damage in the NTS nephritis. Data on (E) serum creatinine levels and (F) the ratio of albumin to creatinine in the urine are shown. Data are expressed as the means±SEM of five to seven animals per group. Morphologic lesions were evaluated by Masson trichrome staining. Treated mice with JQ1 (100 mg/kg per day) displayed fewer and less severe glomerular lesions. G shows a representative animal from each group, and the quantification of the lesion is shown in H. Magnification, ×200 in G; ×400 in G, b. *P<0.05 versus control; ^#P<0.05 versus NTS.