Skip to main content
. 2016 Jul 21;28(2):479–493. doi: 10.1681/ASN.2016010045

Figure 2.

Figure 2.

The levels of miR-146a induced by IL-1β in renal tubular epithelial cells are NF-κB dependent. (A) miR-146a expression after incubation of untreated (white bars) HK-2 cells and cells treated for 24 hours with 50 ng/ml IL-1β (black bars) or 2.5 or 10 μmol/L pharmacologic inhibitor SP600125, SB203580, or TPCA-1. (B) Western blot of p65 NF-κB subunit and β-actin in HK-2 cells after transfection with p65 siRNA or scrambled siRNA (upper panel) and miR-146a expression in transfected untreated HK-2 cells and cells treated with 50 ng/ml IL-1β (lower panel). (C) Activity of the luciferase reporter vector carrying the 600-bp segment of the WT promoter of miR-146a and two NF-κB binding sites compared with a vector containing mismatches in the two NF-κB binding sites (Mut) in HK-2 cells incubated for 24 hours with 50 ng/ml IL-1β. Data are shown as means±SEM of two to three independent experiments. *P<0.05; **P<0.01.