. Author manuscript; available in PMC: 2018 Jan 1.

Published in final edited form as: J Thromb Haemost. 2016 Nov 26;15(1):187–193. doi: 10.1111/jth.13514

Table 1.

Instrument qualification according to resolution and background noise.

	Instrument type	SSI mean [min– max]	BNR mean [min–max]	Qualified instruments
SSC instruments	Accuri C6	0	ND	0/2
	Apogee A50^a	7.7	0.03	1/1
	FACSAria	5.2 [2.8–7.1]	0.07 [0–0.22]	4/5
	FACSCalibur	4.6 [3–6.5]	0.58 [0–2.59]	5/6
	FACSCanto	4.5 [3.4–7]	0.07 [0.01–0.2]	11/11
	FACSVerse	7.1 [7–7.3]	0.12 [0.06–0.19]	2/2
	Influx^a	2.4	0.43	0/1
	LSR Fortessa	8 [7.2–9.1]	0.02 [0.02–0.05]	3/3
	LSR II	7.9 [5.7–10]	0.02 [0–0.03]	4/4
	Stratedigm^a	4.3	0.02	1/1
FSC instruments	Apogee A50^a	11.3	0	1/1
	Epics XL	0	ND	0/1
	FC500	0	ND	0/2
	Guava	0	ND	0/2
	Influx^a	11.3	0.01	1/1
	Navios/Gallios	5.6 [2.8–7.6]	1.53 [0–6.2] (0.03)^b	7/12 (12/12)^b
	Stratedigm^a	4	1	1/1
Total				39/52 (44/52)^b

Instruments tested both in SSC and FSC;

Results after flow cell wash;

Sensitivity index (SSI) = (Median bead A− Median bead B)/ (SD bead A+ SD bead B) where bead A = 0.2µm and bead B = 0.16µm for SSC FCMrs, and bead A = 0.5 µm and bead B = 0.3 µm for FSC FCMrs. SSI > 3 was required to be compatible with the standardization strategy. Background noise ratio (BNR) is the ratio between the number of events per second measured in the protocol settings and the instrument specific maximal number of events per second keeping abort rate at a low level (FACSCanto I/II = 4000, FACSCalibur = 2000, FACSAria I/II = 4000, LSRII (+/− Fortessa) = 4000, FACSVerse = 4000, Apogee A50 = 2000, Stratedigm = 4000, Gallios/Navios = 5000, Influx = 15 000, defined according to both instrument specifications and core lab validation).