Table 1.

Comparison of whole blood thrombin generation, in vivo and ex vivo clot formation in WT, LV17/18^tg and FVIII^null mice.

		WT	LV17/18tg	FVIIInull
nWB-TGA	Lag time (min)	9.1 ± 1.4 *	13.8 ± 1.5	55.6 ± 27.0 **
	Peak time (min)	15.1 ± 2.6 *	23.5 ± 2.0	61.7 ± 22.8 **
	Peak thrombin (nM)	216 ± 41	195 ± 32	9.9 ± 10.7 **
	ETP (nM)	1718 ± 110	1642 ± 139	115.7 ± 155.4 **
	TGR (nM/min)	38.3 ± 15.7	29.0 ± 7.7	1.3 ± 1.2 **
	N (#)	7	6	15
Ex vivo thrombus formation	CT (sec.)	424 ± 54 *	705 ± 91	6292 ± 709 **
	CFT (sec.)	180 ± 45	225 ± 54	950, 995, X, X
	MCF	51.3 ± 3.7	54.0 ± 4.1	X
	alpha angle (°)	58.1 ± 5.9	52.0 ± 6.5	X
	N (#)	7	6	4
In vivo thrombus formation	TTO (min)	6.7 ± 1.6	10.6 ± 3.0	>45 **
	N (#)	4	4	3

Thrombin generation in whole blood of WT, LV17/18^tg and FVIII^null mice was assessed using nWB-TGA. The five thrombin generation parameters lag time, peak time, peak thrombin, endogenous thrombin potential (ETP) and thrombin generation rate (TGR) are listed for indicated mice. Ex vivo clot formation in whole blood from WT, LV17/18^tg and FVIII^null mice was evaluated with ROTEM. Clotting time (CT), clot formation time (CFT), maximum clot firmness (MCF) and the alpha angle are listed. Data from single animals are shown for CFT of FVIII^null mice because 2 mice had not determinable (X) MCF in the 90 min assay. Time to occlusion (TTO) of carotid artery was determined upon ferric chloride induced vessel injury in indicated mice using a Doppler ultrasound flow probe.

^*P < 0.05 for comparison of WT and LV17/18^tg mice.

^***P < 0.001 for comparison of FVIII^null and LV17/18^tg mice.

The Mann-Whitney test, which allows analysis of data with infinite read times scored at the maximum observation period (90 min for lag and peak time, 45 min for TTO) was used for comparisons of nWB-TGA lag and peak time and in vivo clot formation TTO between FVIII^null and LV17/18^tg mice. For all other comparisons the Student’s t-test was used.