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. 2017 Jan 30;3:16107. doi: 10.1038/cddiscovery.2016.107

Figure 2.

Figure 2

Detection of the expression of IAPP and its receptors in NP cells. (a) IAPP and Calcr mRNA levels in normal and degenerated IVD NP cells were determined by RT-qPCR. (b) Statistical analysis showing mRNA expression of IAPP in different groups. (c) Statistical analysis showing mRNA expression of Calcr. (d–f) Statistical analysis showing mRNA expression of RAMP1, RAMP2, and RAMP3. (g) Protein expression of Calcr and RAMP3 in NP cells was determined by western blotting. (h) Statistical analysis showing the relative expression of Calcr protein. (i) Statistical analysis showing the relative expression of RAMP3 protein. (j–l) Statistical analysis showing mRNA expression of IAPP in NP cells infected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2- Puro-IAPP, or treated with pramlintide. (m) Protein expression of Calcr and RAMP3 in NP cells of different groups were determined by western blotting. (n) Statistical analysis showing the relative expression of RAMP3 protein. (o) Statistical analysis showing the relative expression of Calcr protein. Data were shown as means±S.D. *P<0.05, **P<0.01, compared with the normal group.