Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 30;3:16107. doi: 10.1038/cddiscovery.2016.107

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

The effects of IAPP on ECM anabolic gene expression and pro-inflammatory factor secretion in NP cells. Immunocytochemistry assay revealed IAPP protein expression in NP cells infected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2-Puro-IAPP, or treated with pramlintide. (a) Immunocytochemistry assay revealed IAPP protein expression in NP cells after transfection. (b) RT-PCR showed the expression of IAPP in NP cells in different groups. (c) MTT assay showed higher cell viability resulting from the upregulation of IAPP. (d) Glucose uptake measurement. E-H. RT- PCR showed gene expression of aggrecan (e), Col2 (f), and downregulation of TNF-α (g) and IL-1 (h) in different groups. (i and j). ELISA analysis showed protein expression of IL-1 (i) and TNF-α (j) in human NP cells of different groups. *P<0.05, **P<0.01, compared with NC.