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. 2017 Jan 31;8:13. doi: 10.3389/fneur.2017.00013

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Copyright © 2017 Kim, Miller, Lins, Han, Keiser, Boudreau, Davidson and Narayanan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RNA interference of α-synuclein. (A) Design of RNAi molecules based on limited potential off-targeting revealed eight possible sequences, labeled miSyn1–8 (see Tables 1 and 2). miSyn1, 2, 3, 4, and 8 targeted to hSNCA140 and splice variants hSNCA126, 112, and 98. (B) In HEK293 cells, western blotting revealed marked knockdown of hSNCA for miSyn3–5; n = 6 samples per lane. Scrambled RNAi used a positive control. (C) miSyn4 knocked down hSNCA in vivo by qPCR; n = 8 samples. (D) miSyn4 did not affect mouse SNCA by qPCR; n = 9 samples. *p < 0.05 by t-test.