Table 1.

Primer sequences used in nested reverse transcription polymerase chain reaction (RT-PCR) protocol and conventional RT-PCR protocol.^∗

Primer name (Reference)	nt position (referred to AJ608288)	5′→3′ sequence (sense)	Fragment length, bp	Annealing temperature
DMV-2 (Belliere et al., 2011)	15702–15684	ATHCCCAGCTTTGTCTGGT	cDNA production
DMV-11F (Mazzariol et al., 2016)	7799–7819	CCGAACCTGATGATCCATTT	612	56°C
DMV-11R (Mazzariol et al., 2016)	8411–8391	CGTAAATGTCCATCCCTGCT
DMV-13F (Centelleghe et al., 2016)	8052–8072	CATCATAGGGGGTGGTTTGA	200	62°C
DMV-13R (Centelleghe et al., 2016)	8232–8212	GGGGTGGTCTACTCTTGCAC
DMV-1F (This study)	72–92	TCAATTGGCACAGGATTTGG	474	56°C
DMV-1R (This study)	545–525	CCAATGGGTTCCTCTGGTGT
DMV-2F (This study)	501–521	TCTATTCAAGCAGGGGAGGA	622	56°C
DMV-2R (This study)	1122–1102	TCGGCTGTGATCCCTAGTTC
DMV-N1 (Belliere et al., 2011)	1203–1222	CAAGAGATGGTCAGGAGATC	1358	56°C
DMV-P2 (Belliere et al., 2011)	2541–2521	GACAGGTGGTGCAACCCGAC
DMV-C (Belliere et al., 2011)	2132–2152	ATGTTTATGATCACGGCGGT	769	56°C
DMV-4R (This study)	2900–2880	AGGTGGCCTTCGATAGTTGA
DMV-5F (This study)	2439–2459	ACCAATTCCAACCTCAGTGC	716	56°C
DMV-5R (This study)	3154–3134	ATCCCACAGCAGAGCTCATT
DMV-14F (This study)	3037–3057	CCAGCAGTCGAGAGAAATCC	723	56°C
DMV-14R (This study)	3759–3739	TCTCATTTAACCCCGCTGTC

^∗nt, nucleotide; bp, base pair.