Table 1.
TdWRKY gene models and comparaisons with homologous sequences in grass species using Blastp and Blastn.
| BLASTP | BLASTn | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BAC clone ID | T.turgidum predicted gene | Protein Acc.No | Identity (%) | Score | Expect | cDNA Acc.No | Identity (%) | Score | E-value | ||
| TD14H23 | Known_function, WRKY DNA binding domain 3534bp 1407bp 469aa CAT01 |
T. aestivum | ACD80362.1 | 98 | 953 | 0.0 | T. aestivum | EU665430.1 | 99 | 2521 | 0.0 |
| T. urartu | EMS57536.1 | 94 | 811 | 0.0 | – | – | – | – | – | ||
| H. vulgare | BAJ96672.1 | 78 | 755 | 0.0 | H. vulgare | AK365469.1 | 93 | 1625 | 0.0 | ||
| B. distachyon | XP_003562765.1 | 70 | 635 | 0.0 | B. distachyon | XM_003562717.3 | 86 | 1137 | 0.0 | ||
| A. thaliana | NP_178433.1 | 45 | 228 | 1e-67 | – | – | – | – | – | ||
| TD16L16 | Putative_function, Putative WRKY transcription WRKY 3052bp 1716bp 572aa CAT04 |
T. urartu | EMS51071.1 | 93 | 1124 | 0.0 | B. distachyon | XM_010236958.1 | 86 | 522 | 5e-69 |
| A. taushii | EMT16491.1 | 88 | 1010 | 0.0 | – | – | – | – | – | ||
| B. distachyon | XP_010235260.1 | 53 | 536 | 2e-180 | – | – | – | – | – | ||
| A. thaliana | NP_200438.1 | 30 | 199 | 9e-55 | – | – | – | – | – | ||
| TD493B21 | Putative function, WRKY DNA binding domain 1227bp 882bp 294aa CAT01 |
T. aestivum | ABN43185.1 | 99 | 605 | 0.0 | T. aestivum | EF368364.1 | 99 | 1591 | 0.0 |
| T. aestivum | AGF90798.1 | 98 | 602 | 0.0 | S. italica | XM_004962359.3 | 79 | 503 | 3e-138 | ||
| A. tauschii | EMT33135.1 | 87 | 508 | 2e-176 | T. aestivum | EF368357.1 | 92 | 1151 | 0.0 | ||
| A. thaliana | AAM34736.1 | 54 | 278 | 5e-89 | T. aestivum | EU665443.1 | 91 | 1138 | 0.0 | ||
| TD473J01 | Putative_function, WRKY transcription factor 4295bp 1053bp 351aa CAT04 |
T. aestivum | AFW98256.1 | 100 | 724 | 0.0 | T. aestivum | JX679079.1 | 99 | 1940 | 0.0 |
| H. vulgare | CAD60651.1 | 91 | 626 | 0.0 | H. vulgare | AK360269.1 | 92 | 1487 | 0.0 | ||
| S. purpurea | AJF34891.1 | 81 | 554 | 0.0 | H. vulgare | AY541586.1 | 94 | 1367 | 0.0 | ||
| B. distachyon | XP_003570741.1 | 70 | 437 | 3e-149 | B. distachyon | XM_003570693.3 | 80 | 734 | 0.0 | ||
| A. thaliana | NP_178199.1 | 36 | 198 | 9e-60 | – | – | – | – | – | ||
| TD315C07 | Domain_containing_protein, WRKY DNA binding domain 2157bp 1107bp 369aa CAT02 |
T. aestivum | CDM83596.1 | 100 | 751 | 0.0 | H. vulgare | AK358052.1 | 92 | 1507 | 0.0 |
| H. vulgare | BAJ98040.1 | 89 | 511 | 1e-177 | T. aestivum | HG670306.1 | 99 | 1011 | 0.0 | ||
| B. distachyon | XP_003569516.1 | 70 | 356 | 1e-116 | B. distachyon | XM_003569468.3 | 82 | 861 | 0.0 | ||
| A. thaliana | NP_174279.1 | 46 | 173 | 3e-50 | |||||||
| TD789O23 | Gene fragment, WRKY DNA binding domain 683bp 402bp 134aa CAT04 |
T. aestivum | CDM83596.1 | 98 | 285 | 3e-85 | T. aestivum | HG670306.1 | 93 | 990 | 0.0 |
| H. vulgare | BAJ98040.1 | 91 | 177 | 4e-51 | H. vulgare | AK358052.1 | 92 | 785 | 9e-162 | ||
| B. distachyon | XP_003569516.1 | 74 | 100 | 4e-22 | B. distachyon | XM_003569468.3 | 78 | 246 | 4e-61 | ||
| Gene fragment, WRKY DNA binding domain 1567bp 861bp 287aa CAT03 |
T. aestivum | CDM83596.1 | 98 | 375 | 1e-125 | H. vulgare | AK358052.1 | 93 | 1016 | 0.0 | |
| H. vulgare | BAJ98040.1 | 91 | 352 | 8e-117 | T. aestivum | HG670306.1 | 94 | 1689 | 0.0 | ||
| B. distachyon | XP_003569516.1 | 74 | 273 | 2e-85 | B. distachyon | XM_003569468.3 | 84 | 584 | 9e-163 | ||
| A. thaliana | NP_193551.1 | 75 | 174 | 5e-51 | – | – | – | – | – | ||
The genes models were defined on the table using the genomic sequence length (bp), the CDS length (bp), the protein length (aa) and the categories (CAT) as a quality index for the annotator (CAT01, CAT02, and CAT03 correspond to biological evidence found on Triticum and Aegilops Full-length cDNAs, Poaceae Full-length cDNAs and CDS from O. sativa (IRGSP) and B. distachyon genomes annotation, respectively, the genes models predicted by EuGene belong to CAT04.