Table 2.
Putative cis-regulatory elements in the TdWRKY promoter regions.
| Cis-elements | Sequences | Conditions | References | Cis-regulatory elements numbers | Average frequency of cis-elements in WRKY genes promoters | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TdWRKY1 | TdWRKY2 | TdWRKY3 | TdWRKY4 | TdWRKY5 | AtWRKY | TaWRKY | HvWRKY | ||||
| ABRE | YACGTGKC | ABA, dehydration, salt | Yamaguchi-Shinozaki and Shinozaki, 2005 | 5 | 0 | 6 | 6 | 5 | 0 | 2.2 | 3.4 |
| ARF | TGTCTC | Auxin | Hagen and Guilfoyle, 2002 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
| CBH-HV | RYCGAC | Dehydration, Cold | Svensson et al., 2006 | 4 | 0 | 4 | 0 | 0 | 0 | 0.6 | 1.4 |
| GCC-box | GCCGCC | Ethylene, dehydration | Wu et al., 2007 | 5 | 0 | 0 | 6 | 0 | 0.73 | 3.8 | 3 |
| LTRE | CCGAC | Cold | Baker et al., 1994 | 6 | 1 | 5 | 2 | 3 | 0 | 1.8 | 1.4 |
| MYB | YAACKG | ABA, dehydration salt | Abe et al., 1997 | 12 | 12 | 11 | 12 | 12 | 1.47 | 5 | 6.2 |
| MYC | CANNTG | ABA, dehydrationsalt | Abe et al., 1997 | 11 | 13 | 5 | 5 | 7 | 0 | 3.6 | 0 |
| RAV | CAACA | Ethylene | Kagaya et al., 1999 | 8 | 9 | 2 | 4 | 3 | 0 | 2.8 | 0.4 |
| Wbox | TGAC | Biotic and abiotic stress | Eulgem et al., 1999, 2000 | 6 | 5 | 14 | 12 | 15 | 4.4 | 4.2 | 4.6 |
| DRE/CRT | R(A/G)CCGAC | Dehydration, Cold | Dubouzet et al., 2003; Skinner et al., 2005 | 4 | 0 | 3 | 0 | 1 | 0 | 1.2 | 2.6 |
The 1.5 Kb DNA fragments corresponding to the 5′ -regulatory regions upstream of the ORF (Open Reading Frame) transcription start sites of TdWRKY genes were subjected to an in silico analysis using a PLACE signal scan and NSITE-PL to search for putative cis-regulatory motifs potentially involved in the control of TdWRKYgenes expression. R, A/G; Y, C/T; K, G/T; N, any nucleotide. Frequencies of potential binding sites in AtWRKY promoters were derived from the study of Dong et al., 2003.