FIGURE 4.

In situ hybridization of the VviFSEX gene in several flower developmental stages in three Vitis flower types. The transcript of VviFSEX gene is present in early flower developmental stages in male (A), female (B) and hermaphrodite (C) plants when the floral primordia display emerging sepals and/or petals. When stamens are visible VviFSEX is expressed in petals, stamens and carpel region, throughout the three flower types (D–F). Later, the gene transcript remains in stamens and in the carpel but seems to be absent from petals (G–I). Cutting planes are represented in (J–L) with the respective slide section outlined. Cuts were performed with 7 μm thick. Flowers structures: se, sepals; pt, petals; st, stamens; c, carpel. Scale bar = 100 μm.