Table 1.
MBL2 SNP | Primer type | Sequence | Primer conc. used (p mol/μl) | Product size (bp) |
---|---|---|---|---|
rs11003125 | H specific forward primer | 5′-GCTTACCCAGGCAAGCCTGTG-3′ | 0.1 | 316 |
L specific forward primer | 5′-GCTTACCCAGGCAAGCCTGTC-3′ | 0.1 | ||
Reverse common primer | 5′-AACAAATGGGACCGTGCATTGC-3′ | 0.05 | ||
rs7096206 | Forward common primer | 5′-CCTGCCAGAAAGTAGAGAGG-3′ | 0.15 | 440 |
Y specific reverse primer | 5′-CTGGAAGACTATAAACATGCTTTCC-3′ | 0.15 | ||
X specific reverse primer | 5′-GGAAGACTATAAACATGCTTTCG-3′ | 0.15 | ||
rs7095891 | P specific forward primer | 5′-CAGATTGTAGGACAGAGGGCATGCTC-3′ | 0.05 | 332 |
Q specific forward primer | 5′-TTGTAGGACAGAGGGCATGCTT-3′ | 0.1 | ||
Reverse common primer | 5′-CCAGGCAGTTTCCTCTGGAAGG-3′ | 0.05 | ||
rs1800450 | Forward common primer | 5′-CTGCACCCAGATTGTAGGACAGAG-3′ | 0.1 | 278 |
A specific reverse primer | 5′-CCCCCTTTTCTCCCTTGGTGC-3′ | 0.1 | ||
B specific reverse primer | 5′-CCCCCTTTTCTCCCTTGGTGT-3′ | 0.1 |
The nucleotides defining the polymorphisms are underlined.