Skip to main content
. 2017 Jan 31;8:75. doi: 10.3389/fmicb.2017.00075

Table 1.

Oligonucleotide primers used for MBL2 genotyping by ARMS-PCR.

MBL2 SNP Primer type Sequence Primer conc. used (p mol/μl) Product size (bp)
rs11003125 H specific forward primer 5′-GCTTACCCAGGCAAGCCTGTG-3′ 0.1 316
L specific forward primer 5′-GCTTACCCAGGCAAGCCTGTC-3′ 0.1
Reverse common primer 5′-AACAAATGGGACCGTGCATTGC-3′ 0.05
rs7096206 Forward common primer 5′-CCTGCCAGAAAGTAGAGAGG-3′ 0.15 440
Y specific reverse primer 5′-CTGGAAGACTATAAACATGCTTTCC-3′ 0.15
X specific reverse primer 5′-GGAAGACTATAAACATGCTTTCG-3′ 0.15
rs7095891 P specific forward primer 5′-CAGATTGTAGGACAGAGGGCATGCTC-3′ 0.05 332
Q specific forward primer 5′-TTGTAGGACAGAGGGCATGCTT-3′ 0.1
Reverse common primer 5′-CCAGGCAGTTTCCTCTGGAAGG-3′ 0.05
rs1800450 Forward common primer 5′-CTGCACCCAGATTGTAGGACAGAG-3′ 0.1 278
A specific reverse primer 5′-CCCCCTTTTCTCCCTTGGTGC-3′ 0.1
B specific reverse primer 5′-CCCCCTTTTCTCCCTTGGTGT-3′ 0.1

The nucleotides defining the polymorphisms are underlined.