FIGURE 6.

Anti-sense of AaHDR1 leading to decrease of artemisinin in leaves of transgenic plants. (A) a cassette scheme shows anti-sense orientation in plasmid PMDC-antiAaHDR1 to down-regulate AaHDR1 expression; (B) phenotypes of two transgenic lines vs. wild-type plants after grown on pot soil for 6 weeks; (C) RT-PCR images show reduced expression levels of AaHDR1 in young leaves of 6-week old transgenic plants; (D) western blot images show decrease of protein level; (E) contents of artemisinin is reduced in two transgenic lines. Bars, which are labeled with different low case letters for each metabolite, mean significant difference (p-values < 0.05), while, bars, which are labeled with the same low case letters, mean insignificant difference. AS-14 and 15, two anti-sense transgenic plants; WT, wild-type.