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. 2017 Jan 31;10:24. doi: 10.3389/fnmol.2017.00024

Figure 3.

Figure 3

Mosaic Expression using Differentially Localized Reporters (MEDLR) is a novel method to compare WT and KD neurons on the same coverslip. (A) MEDLR uses combinatorial lentiviral transgenesis with memGFP lentivirus and nlsGFP-linked amiRNA lentivirus to compare WT and KD cells. (B) Representative immunolabled GFP images at 20× or (C) 60× magnification of memGFP-labled amiRNA− and amiRNA+ neurons at 15 DIV in cultures transduced with sub-saturating amounts of Scrambled1-3 amiRNAs. (D) Representative 40× images of live GFP fluorescence in 15 DIV amiRNA− and amiRNA+ neurons. (B–D) Note the perisynaptic memGFP accumulation that could potentially be misinterpreted as nlsGFP in amiRNA− cells. (B) Scale, 50 μm; (C,D) Scale, 10 μm.