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. 2017 Jan 31;10:10. doi: 10.3389/fnmol.2017.00010

Figure 2.

Figure 2

MTT vs. Trypan blue exclusion assay: Comparative effect of Cu and/or PA on viability of U251 cells. U251 Cells (0.8 × 104) were treated with CuSO4 (50 μM, 200 μM), PA (250 μM), or Cu-PA (50–250 μM) for 24 h. Data presented as % viability is the mean ± SEM of 18-determinations from 6-different experiments using MTT assay, and of 3-determinations from 3- different experiments using Trypan blue exclusion assay. Asterisks on bars represent inter-categorical statistical significance (each category with the preceding one), and those drawn upwards represent significance relative to the control. (***) correspond to P < 0.001.