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The ps:LC neurons form a distinct module. Paired injections of CAV2-PRS-mCherry-ChR2 and CAV2-PRS- EGFP-2A-PSAM to the lumbar spinal dorsal horn and prefrontal cortex (CG1), respectively, labeled distinct subsets of LC neurons (n=3 rats) with the spinal group (red) located more ventrally in the LC with only minimal overlap with those neurons labeled from frontal cortex (green, arrow marks a single double labeled cell body). DBH-ir – revealed with AMCA secondary antibody (blue).
