Fig. 8.

ps:LC neurons have distinctive electrophysiological characteristics. (A) Whole cell recordings from retrogradely transduced ps:LC neurons showed they could be opto-activated (20 ms×10 mW, 25 Hz, expanded inset). (B) Recordings from neurons in ps:LC and in the core of the LC; both showing healthy patterns of activity. (C) Action potential morphologies from two representative neurons (green – ps:LC, blue – LC core) and scatter plot of action potential duration plotted against AHP amplitude for each LC neuron showing that ps:LC neurons (green, n=9) had significantly shorter spike durations and smaller AHPs then neurons in the core of the LC (blue, n=24). (**** - P<0.0001; *** - P<0.001, unpaired Student׳s t-test).