Table 2.

Quantitation of retrograde labeling of pontospinal noradrenergic neurons by CAV2-PRS-ChR2-mCherry.

Cell count in each pontine nucleus	Medium titre CAV2 (0.9×1010 TU/ml)	High titre CAV2 (1.2×1012 TU/ml=2.6×1012 pp/ml)
LC	360±115 (75%)	546±80 (74%)
A5	31±10 (7%)	102±66 (14%)
A7	87±48 (18%)	85±26 (12%)
Total in Pons	479±161	733±170

Vector injections (4×500 nl, two per side) made to lumbar dorsal horn in segments L3/4. (Mean±SEM, n=3 rats per titre, proportion of the total number of retrogradely labeled NA neurons given per nucleus in brackets).