Figure 1.

PGRN inhibits the proliferation of stimulated human PBMCs. hPBMCs were stimulated by PHA and incubated with PGRN at concentrations of 2, 20, and 200 ng/ml for 5 days (a). Isolated CD4⁺ T lymphocytes were incubated with PHA in the presence of PGRN at concentration of 2, 20, and 200 ng/ml for 5 days (b). Isolated CD3⁺ T lymphocytes were incubated with PHA in the presence of 200 ng/ml of PGRN (c). EdU was added to the culture medium 18 hr before cell harvest. Cells that had incorporated EdU were stained with Alexa Flour 488 and counted by flow cytometry. Data are presented as the means ± standard deviations of three experiments performed in triplicate. ^∗∗p < 0.01, ^∗∗∗p < 0.001. Similar results were obtained in four independent experiments.