Figure 3.

Hypoxia inhibited Wnt/β-catenin pathway. Expressions of Wnt4 and downstream molecules in the Wnt pathway, β-catenin, LEF1, and Axin2, were measured by western blotting and/or RT-qPCR, 3 days after hypoxic or normoxic culture. Hypoxia decreased the expression of these molecules. (a)–(d) Protein levels of Wnt4, β-catenin, and LEF1 were decreased by hypoxic culture; ^∗p < 0.05; ^∗∗p < 0.01; n = 6. (e)–(h) RNA levels of Wnt4, β-catenin, LEF1, and Axin2 were also reduced by hypoxic culture; ^∗p < 0.05; ^∗∗∗p < 0.001; n = 3. Data are represented as mean ± SD.