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. 2017 Jan 31;7:41764. doi: 10.1038/srep41764

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(a) Hoechst³³³⁴² staining reveals the nuclear chromatin. (b) IRIF are visualized with γH2AX immuno-detection in fixed cells exposed to ²³⁹Pu source. (c) GFP-RNF8 is re-localized to the DNA damaged areas, and (d) the merged image shows the overlap of GFP-RNF8 and γH2AX 30 minutes after irradiation.