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. 2017 Jan 31;7:41551. doi: 10.1038/srep41551

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Post thawing cells were cultured in a CO₂ incubator for 7 days (37 °C, 5% CO₂). Cell proliferation assay of cells seeded gelatin cryogel (Ab) without cryopreservation (control), (Bb) 1 day and (Cb) 1 month cryopreservation. Nuclei staining at day 1 (Aa,Ba,Ca) and at day 7 (Ac,Bc,Cc) in case of control, after 1-day, and 1-month cryopreservation, respectively. Nuclei were stained with DAPI (blue). Scale bar: 200 μm; magnification: 100×.