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. 2017 Jan 31;7:41715. doi: 10.1038/srep41715

Figure 1. Concurrent knockdown of Akt1 and IKKβ down-regulates histone H4 expression.

Figure 1

(a) A549 and H2009 cells were transfected with Akt1-, IKKβ-siRNA or both. Negative control (NC) siRNA transfected cells were used as a negative control. The knockdown efficacy of IKKβ and Akt1 was confirmed by Western blotting. GAPDH was used as a loading control. (b) Reduced histone H4 mRNA expression was detected by qRT-PCR. H2AB was detected as a negative control (*p < 0.05; **p < 0.01). (c) The expression of H4 protein was detected by Western blot. Lamin B1 was detected as a loading control.