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. 2017 Jan 31;7:41715. doi: 10.1038/srep41715

Figure 4. Histone H4 suppresses proteasomal degradation of RIP1 and IAPs induced by cisplatin.

Figure 4

(a) The cells were treated with cisplatin (7.5 μM) for indicate times, and the expression of the indicated proteins was detected by Western blot. GAPDH was detected as a loading control. (b) The cells were treated with cisplatin (7.5 μM) for 2 h. The expression of the indicated proteins was detected by Western blot. GAPDH was detected as a loading control. (c–g) The cells were pretreated with zVAD (20 μM), MG132 (10 μM), chloroquine (CQ, 20 μM), NAC (1 mM) or BHA (100 μM) for 1 h. Then the cells were treated with cisplatin (7.5 μM) for another 2 h. The expression of the indicated proteins was detected by Western blot. GAPDH was detected as a loading control.