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. 2017 Jan 31;7:41715. doi: 10.1038/srep41715

Figure 5. Suppression of cisplatin-induced DNA-PK activation contributes to RIP1 and IAPs downregulation in H4 knockdown cells.

Figure 5

(a,b) The cells were pre-treated with DNA-PK inhibitor NU7026 (10 μM) for 1 h or left untreated and treated with cisplatin (7.5 μM) treatment for another 72 h. Cell death was measured by LDH releasing assay. Columns, mean of three experiments; bars, SD. **p < 0.01. (c) The cells were treated with cisplatin (7.5 μM) for 30 min. The expression of the indicated proteins was detected by Western blot. GAPDH was detected as a loading control. (d,e) The cells were pre-treated with NU7026 (10 μM) for 1 h or left untreated followed by cisplatin (7.5 μM) treatment for another 2 h. The expression of the indicated proteins was detected by Western blot. GAPDH and Lamin B1 were detected as a loading control.