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. 2017 Jan 31;7:41701. doi: 10.1038/srep41701

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Experimental scheme for assessing proliferation of NPCs in vitro. (b) Experimental scheme for assessing differentiation of NPCs in vitro. (c) Adult hippocampal NPCs culture under proliferating conditions expressed the neural progenitor cell marker Nestin (cytoplasmic, red); MeCP2 in green; Dapi in blue. Scale bar: 100 μm. (d) Both WT and TG NSCs incorporate the thymidine analog, BrdU, under proliferating conditions (BrdU, red). Scale bar: 100 μm. (e) Differentiation of adult NSCs into neurons (Tuj-1, green) by 1 μM RA and 5 μM forskolin. Scale bar: 100 μm. (f) Differentiation of adult NSCs into astrocytes (GFAP, red) by 1% FBS. Scale bar: 100 μm. (g–i) Quantitative analysis showing the percentages of BrdU⁺, Tuj-1⁺, and GFAP⁺ cells in WT and TG individuals respectively. Values are Mean ± S.E.M (ns: non-significant, student’s t-test).