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. 2017 Jan 31;7:41510. doi: 10.1038/srep41510

Figure 1. Deferasirox induces death of proximal tubular epithelial cells.

Figure 1

Tubular cells were exposed to the indicated concentrations of deferasirox for different times. (A) Analysis of tubular cell viability at 24 hours by MTT assay. Mean ± SEM of three independent experiments. *p < 0.001 vs control. (B) Contrast phase microscopy photographs (original magnification x200). An example representative of three independent experiments is shown. Note dose-dependent cell detachment. Scale bars: 200 μm. (C) Cells were exposed to deferasirox for 24 h, stained with annexin V/7-AAD and analyzed by flow cytometry. Deferasirox increased both annexin V+/7-AAD− cells and annexin V+/7-AAD+ cells. Mean ± SEM of three independent experiments. *p < 0.04 vs control. (D) Time-course of annexin V staining in tubular cells exposed to 10 μM deferasirox. Mean ± SEM of three independent experiments. *p < 0.01 vs control; #p < 0.02 vs deferasirox 24 h. (E) Hypodiploid cells, suggestive of apoptosis, were assessed by flow cytometry of DNA content. Deferasirox increased the percentage of hypodiploid cells. Mean ± SEM of three independent experiments; *p < 0.0001 vs control 24 h. *p < 0.02 vs control 48 h.