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. 2017 Jan 31;7:41510. doi: 10.1038/srep41510

Figure 7. Deferasirox-iron complex does not induce tubular cell death.

Figure 7

Tubular cells were exposed to different concentrations of deferasirox-iron complex for 24 h. (A) Viability was assessed by MTT assay. Deferasirox-iron complex was not toxic in tubular cells. Mean ± SEM of four independent experiments. (B) Cell death was assessed by annexin V/7-AAD staining and flow cytometry in tubular cells treated with deferasirox or deferasirox-iron complex. Mean ± SEM of four independent experiments. *p < 0.03 versus control, #p < 0.001 vs deferasirox. (C) Tubular cells treated with deferasirox-iron complex do not present pyknotic nuclei and ramain attached to the plate. DAPI staining. Magnification x200. Scale bars: 50 μm. (D) Deferasirox-iron complex does not induce MMP loss in tubular cells. Graph shows TMRM staining assessed by flow cytometry. Mean ± SEM of four independent experiments. *p < 0.03 versus control, #p < 0.001 vs deferasirox.