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. 2017 Jan 31;7:41476. doi: 10.1038/srep41476

Figure 3. Phenanthrene decreases extracellular Ca2+ influx via the L-type Ca2+ current, ICaL in bluefin tuna ventricular myocytes.

Figure 3

(A) L-type Ca2+ channel current (ICaL) density recorded from bluefin tuna ventricular myocytes via whole-cell voltage clamp in control condition (black trace) and with ascending concentrations of phenanthrene (pink/red traces) subsequently perfused over the same cell. (B) Mean data ± SEM (n = 6, N = 2) ICaL amplitude in control (black bar) and with an increasing concentrations of phenanthrene (pink bar, 5 μM; red bar, 25 μM). (C) Change in ICaL amplitude (expressed as a percentage of control) with ascending concentrations of phenanthrene (pink bar, 5 μM; red bar, 25 μM). Similar inhibitory effects were found in two atrial myocytes (not shown). *Indicates significant difference (P < 0.05, Student’s t-test or one-way ANOVA).