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. 2017 Jan 31;7:41862. doi: 10.1038/srep41862

Figure 1. IMB-6G induces cytotoxicity and apoptosis in pancreatic cancer cells.

Figure 1

(a) Structure of the IMB-6G molecule. (b) MiaPaCa-2 and HupT-3 cells were treated with various concentrations of IMB-6G for the indicated time. Cell viability was measured by MTT assay. (c) MiaPaCa-2 and HupT-3 cells were treated with indicated concentrations of IMB-6G for 24 h. The protein expression levels of cleaved caspase 9, cleaved caspase 3, and poly (ADP-ribose) polymerases (PARP) were detected by immunoblotting. (d) MiaPaCa-2 and HupT-3 cells were treated with IMB-6G in the presence or absence of Z-VAD-FMK (20 μM). Cleaved PARP1, caspase 9 and caspase 3 were detected by immunoblotting. (e) MiaPaCa-2 and HupT-3 cells were stained with Annexin V-FITC and PI after incubated with indicated concentrations of IMB-6G or DMSO in the presence or absence of Z-VAD-FMK for 24 h, the numbers of apoptotic cells were analyzed by flow cytometry. Annexin V-positive cells were accepted as apoptotic cells. The results are presented as mean ± SD and represent three individual experiments. *p < 0.05, **p < 0.01 compared with the untreated control group.