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. 2017 Jan 31;7:41862. doi: 10.1038/srep41862

Figure 2. IMB-6G induces autophagosome accumulation in pancreatic cancer cells.

Figure 2

(a) MiaPaCa-2 and HupT-3 cells were transfected with the EGFP-LC3 plasmid. After 24 h, the cells were incubated with IMB-6G (5 μM), CQ (50 μM) or DMSO (Ctrl) for 6 h and visualized with confocal microscopy (upper panel; scale bars, 20 μm). (b) The number of punctate EGFP-LC3 in each cell was counted, and at least 100 cells were included for each group (lower panel). Data were the mean value of three independent experiments with each count of no less than 100 cells.*p < 0.05, **p < 0.01 compared with the untreated control group. (c) MiaPaCa-2 and HupT-3 cells were treated with IMB-6G at the indicated concentrations for 24 h, or treated with IMB-6G (5 μM) for indicated time points, the lipidation of LC3 and the levels of p62/SQSTM1 were detected by immunoblotting using corresponding antibodies.