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. 2017 Jan 31;7:41626. doi: 10.1038/srep41626

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(A) Growth of S. cerevisiae JJ345 cells expressing wild-type (WT) Sgt1 or indicated mutants. Overnight 28 °C SD-Trp cultures were serially diluted, then pinned onto SD-Trp agar and photographed after 2 days of growth at 28 °C and 37 °C. (B) Analysis of Sgt1 level in 28 °C SD-Trp cultures expressing WT Sgt1 and either WT or mutant Sgt1-His₆. Actin was measured as a loading control. Full-length blots are presented in Supplementary Figure S6B.