Skip to main content
. 2017 Jan 31;7:38734. doi: 10.1038/srep38734

Figure 1. Cilia Function is Required for Expression of flp-19::GFP in the BAG Gas-sensing Neurons.

Figure 1

(A) Quantification of the expression of the flp-19::GFP fluorescent reporter (ynIs34) in wild type, che-3(e1379) and tub-1(ok1972) mutant animals. n > 50. ****P < 0.0001. See Materials and Methods for neuronal scoring criteria used. (B) Micrographs show representative pictures of flp-19::GFP(ynIs34) expression in wild type, che-3(e1379) and tub-1(ok1972) strains. BAG positions are marked with red dashed circles. Note - expression of flp-19::GFP is also lost in the ciliated AWA neurons in che-3(e1379) and tub-1(ok1972) mutant animals (blue dotted circles). Anterior to the left. Scale bar = 20 μm. (C) Quantification of the expression of the flp-19::GFP reporter (rpEx811) in wild type and che-3(e1379) backgrounds. These data confirm the results shown in Fig. 1A. n > 50. ****P < 0.0001.