Table 1.

Evolution of sequencing platforms.

	Platforms	Companies	Detection Methods	References	Advantages + / Disadvantages -
Conventional sequencing	Sanger	Sanger biochemistry	Capillary electrophoresis/ clone by clone strategy	[34]	Low throughput-, In vivo cloning,- Long reads+/ High costs-
Next Generation Sequencing	GS FLX	Roche 454 Life Sciences,	Pyrosequencing detection of pyrophosphate	[14]	No in vivo cloning+ Better sequence quality+, High throughput+, Low cost+, wide range of uses+, Very short reads-. Difficulty for genome sequencing assemblies-
	George Church’s Laboratory at Harvard University	Polony sequencing	Multiplex polony sequencing	[35]
	Genome analyzer	Solexa, Illumina Inc	Fluorophore labeled reversible terminator nucleotides	[36]
	Intelligent Bio-Systems	Qiagen	sequencing by synthesis	[37]
	SOLiD (Supported oligo ligation detection)	Applied Biosystems	Fluorophore labeled oligonucleotide probes	[38]
	HeliScope	Helicos Bioscience	Signe molecule detection system, sequencing by synthesis	[15]
	Ion PGM System	Life Technology Personal genome machine	Post-light Ion semiconductor sequencing	[39]
	Ion PGM System	Life Technology Personal genome machine	Non-optical DNA sequencing	[40]
Third Generation Sequencing	PacBio RS	Pacific biosciences	Phospholinked fluorophore labeled nucleotides	[16]	Sequencing in real time+ Long reads length+ novo genome assemblies+
	PacBio RS	Pacific biosciences	specific single-molecule sequencing of 5-hydroxy methylcytosine	[41]