Fig. 1.

OK-432 enhanced antitumor effects after MWA. a Kaplan-Meier curves showing survival of untreated mice and mice treated with OK-432, MWA, and MWA plus OK-432. For the rechallenge test, 2 × 10⁴ 4T1 cells were injected into left inguinal mammary fat pads of surviving mice on day 25 after MWA. Age-matched healthy mice served as control (n = 5). b Tumor volumes of mice that survived over 10 days after rechallenge were calculated every 5 days (Experimental group: n = 6; Control group: n = 5). On day 25 after rechallenge, 5/5 of control mice and 1/6 of MWA plus OK-432 treated mice developed tumors after rechallenge. Points, mean; error bars, SEM. *P < 0.05; ***P < 0.001. One representative experiment out of three is shown