Table 1.
Primers for complete genome amplification of ToMMV
| Amplified fragments | sequence(5’-3’) | direction | site | length(bp) | Tm(°C) |
|---|---|---|---|---|---|
| ToMMV1 | TTTAAGTATTTATTATTACAACAATTA | + | 1 ~ 1465 | 1465 | 54.2 |
| GGACAGAAAGCTTTGTATGTAGG | - | ||||
| ToMMV2 | TTTAAAGTCACCGCTAGGTCTGAGT | + | 1377 ~ 2816 | 1440 | 60.5 |
| CCAGTGTGCAGCATCAATCC | - | ||||
| ToMMV3 | GCTAAGGTTGTACTAGTAGACGG | + | 2551 ~ 3433 | 883 | 60.5 |
| GTAATTGCTATTGAGTACCTGC | - | ||||
| ToMMV4 | TTTAAACAGTCCCCATGTGCTTGTC | + | 3313 ~ 4481 | 1209 | 60.6 |
| CCATCGGTAACATCGAGGCT | - | ||||
| ToMMV5 | TTTAACTGGAGAAGACTGGGTCTAG | + | 4290 ~ 5565 | 1193 | 60.3 |
| TTCGGTAAGTTCAATGGGACCT | - | ||||
| ToMMV6 | TTTAAGGAACATATGGCAAGTCCTAGT | + | 5311 ~ 6398 | 1089 | 60.5 |
| TGGGCCCCTACCGGGG | - | ||||
| 5RAGSPR1 | CAAGCGAGTGAACTGCGTTCTGAGTG | - | 1 ~ 325 | 325 | 69.5 |
| 5RAGSPR2 | GCCCGGGTAGCAATAAGTGTCTGTTC | - | 1 ~ 269 | 269 | 69.5 |
| 3RAGSPF1 | GTTAATGAATTGGTAAGAGGAACAGG TTTC |
+ | 6106 ~ 6398 | 293 | 66.7 |
Annotation: TTTAA was added to the upstream primer according to the requirement of the cloning vector