Table 2.
Cell growth, glucose consumption, ethanol formation, and furan aldehydes conversion of recombinant Z. mobilis strains
| Inhibitors | Recombinant strains | Cell growth (OD600) | Glucose consumption (g/L) | Ethanol production (g/L) | Inhibitor conversion (g/L) |
|---|---|---|---|---|---|
| Furfural | ZM4(pHW20a-gfp) (Control) | 0.361 ± 0.003 | 4.81 ± 0.58 | 1.37 ± 0.02 | 1.13 ± 0.09 |
| ZM4(pHW20a-ZMO0062) | 0.387 ± 0.003 | 4.13 ± 0.26 | 1.36 ± 0.04 | 1.04 ± 0.03 | |
| ZM4(pHW20a-ZMO1696) | 0.404 ± 0.000 | 4.28 ± 0.19 | 1.39 ± 0.02 | 1.18 ± 0.03 | |
| ZM4(pHW20a-ZMO1722) | 0.393 ± 0.004 | 4.78 ± 0.22 | 1.56 ± 0.06 | 1.34 ± 0.06 | |
| ZM4(pHW20a-ZMO1771) | 0.378 ± 0.027 | 4.60 ± 0.26 | 1.49 ± 0.00 | 1.51 ± 0.02 | |
| ZM4(pHW20a-ZMO1993) | 0.359 ± 0.009 | 4.87 ± 0.03 | 1.44 ± 0.00 | 1.02 ± 0.02 | |
| ZM4(pHW20a-ZMO0976) | 0.362 ± 0.007 | 4.09 ± 0.05 | 1.37 ± 0.06 | 0.99 ± 0.05 | |
| ZM4(pHW20a-ZMO1344) | 0.380 ± 0.002 | 4.56 ± 0.04 | 1.58 ± 0.03 | 1.01 ± 0.03 | |
| ZM4(pHW20a-ZMO1673) | 0.314 ± 0.008 | 3.71 ± 0.01 | 1.09 ± 0.20 | 0.93 ± 0.03 | |
| ZM4(pHW20a-ZMO1773) | 0.293 ± 0.002 | 3.19 ± 0.24 | 1.17 ± 0.14 | 0.93 ± 0.16 | |
| ZM4(pHW20a-ZMO1984) | 0.348 ± 0.009 | 4.80 ± 0.09 | 1.39 ± 0.09 | 1.00 ± 0.02 | |
| HMF | ZM4(pHW20a-gfp) (Control) | 0.419 ± 0.005 | 6.47 ± 0.04 | 2.26 ± 0.14 | 1.40 ± 0.09 |
| ZM4(pHW20a-ZMO0062) | 0.439 ± 0.009 | 5.57 ± 0.25 | 2.12 ± 0.17 | 1.43 ± 0.02 | |
| ZM4(pHW20a-ZMO1696) | 0.497 ± 0.014 | 6.48 ± 0.07 | 2.04 ± 0.13 | 1.67 ± 0.08 | |
| ZM4(pHW20a-ZMO1722) | 0.427 ± 0.021 | 6.02 ± 0.04 | 1.89 ± 0.05 | 1.85 ± 0.03 | |
| ZM4(pHW20a-ZMO1771) | 0.471 ± 0.001 | 5.96 ± 0.24 | 2.41 ± 0.01 | 1.89 ± 0.04 | |
| ZM4(pHW20a-ZMO1993) | 0.419 ± 0.001 | 5.92 ± 0.42 | 2.16 ± 0.08 | 1.19 ± 0.08 | |
| ZM4(pHW20a-ZMO0976) | 0.466 ± 0.005 | 6.95 ± 0.04 | 2.38 ± 0.05 | 1.41 ± 0.05 | |
| ZM4(pHW20a-ZMO1344) | 0.396 ± 0.020 | 5.96 ± 0.94 | 2.18 ± 0.04 | 1.65 ± 0.21 | |
| ZM4(pHW20a-ZMO1673) | 0.356 ± 0.013 | 5.44 ± 0.10 | 2.01 ± 0.04 | 1.16 ± 0.01 | |
| ZM4(pHW20a-ZMO1773) | 0.306 ± 0.009 | 5.27 ± 0.45 | 1.78 ± 0.04 | 1.03 ± 0.00 | |
| ZM4(pHW20a-ZMO1984) | 0.400 ± 0.002 | 5.20 ± 0.05 | 2.23 ± 0.03 | 1.55 ± 0.07 |
In RM medium containing 2 g/L of furfural or 4 g/L of HMF at 30 °C. ZM4(pHW20a-gfp) is the control strain only expressing the reporter gene gfp. The fermentation performance was detected at the mid-log phase, where 12 h for furfural and 16 h for HMF. Standard deviations were derived from at least two independent determinations