Table 3.
Furfural and HMF conversion rate of recombinant Z. mobilis strains in corn stover hydrolysate
| Recombinant strains | Furfural conversion (g/L) | HMF conversion (g/L) | ||
|---|---|---|---|---|
| 12 h | 24 h | 12 h | 24 h | |
| ZM4(pHW20a-gfp) (Control) | 0.15 ± 0.01 | 0.33 ± 0.02 | 0.02 ± 0.01 | 0.05 ± 0.01 |
| ZM4(pHW20a-ZMO0062) | 0.15 ± 0.01 | 0.28 ± 0.02 | 0.01 ± 0.00 | 0.01 ± 0.00 |
| ZM4(pHW20a-ZMO1696) | 0.18 ± 0.00 | 0.33 ± 0.02 | 0.02 ± 0.00 | 0.05 ± 0.03 |
| ZM4(pHW20a-ZMO1722) | 0.18 ± 0.04 | 0.32 ± 0.05 | 0.02 ± 0.01 | 0.05 ± 0.01 |
| ZM4(pHW20a-ZMO1771) | 0.26 ± 0.02 | 0.40 ± 0.01 | 0.03 ± 0.01 | 0.07 ± 0.00 |
| ZM4(pHW20a-ZMO1993) | 0.16 ± 0.00 | 0.33 ± 0.01 | 0.01 ± 0.01 | 0.03 ± 0.00 |
| ZM4(pHW20a-ZMO0976) | 0.17 ± 0.02 | 0.31 ± 0.00 | 0.01 ± 0.00 | 0.03 ± 0.02 |
| ZM4(pHW20a-ZMO1344) | 0.14 ± 0.01 | 0.27 ± 0.01 | 0.01 ± 0.00 | 0.03 ± 0.01 |
| ZM4(pHW20a-ZMO1673) | 0.18 ± 0.00 | 0.29 ± 0.03 | 0.01 ± 0.00 | 0.06 ± 0.00 |
| ZM4(pHW20a-ZMO1773) | 0.13 ± 0.00 | 0.23 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.01 |
| ZM4(pHW20a-ZMO1984) | 0.20 ± 0.02 | 0.31 ± 0.04 | 0.02 ± 0.00 | 0.04 ± 0.02 |
The corn stover hydrolysate contained 59.45 g/L of glucose, 24.03 g/L of xylose, 3.21 g/L of acetic acid, 0.38 g/L of HMF and 0.69 g/L of furfural at 30 °C. Standard deviations were derived from at least two independent determinations